Dr. Hattem Mohamed El-Shabrawi (phd)
Post doctorate
Signaling Peptide Lab, Email: helshabrawi73@yahoo.com,
Center for Signal Transduction elshabrawi73@gmail.com
& Metabolomics (C-STM), Mobile: +86-13671334248
Institute of Botany, Chinese Academy of Science, Lab: +86-10-62836943
20 Nanxincun, Xiangshan, Beijing 100093, China. Fax: 86-10-82599701
http://cstm.ibcas.ac.cn/
Research Interests:
My research interest includes Developmental biology, Functional genomics and Signal transduction.
Education:
2003- 2007: Ph.D., pre-doctoral fellow (Plant Molecular Biology group) International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
1998-2002: M.Sc in Plant Biotechnology, Genetic Division, Botany Department, Faculty of Agriculture, Al-Azher University, Cairo, Egypt.
Research topic: “Studies on variation of plant cells and tissue culture on Egyptian chicory Cichorium indiva L. supsp. pumilum(Jacq.).
1990-1995: B.Sc., Genetics Division, Botany Department,Faculty of Agriculture, Al- Azher University, Cairo, Egypt.
Research Experience:
June 2008 - till date: Post doctorate in Signaling Peptides lab, center for Signal Transduction
& Metabolomics (C-STM), Institute of Botany, Chinese Academy of Science,
20 Nanxincun, Xiangshan, Beijing 100093, China
September 2003 - 2007: Pre doctoral fellow in Plant Molecular Biology group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
April 2008 – till date: Researcher in Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre (NRC), Gizza, Egypt.
August 2002 – Marsh 2008 : Associate researcher in Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre(NRC), Gizza, Egypt.
September 1998 – July 2002: Researcher assistant in Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Center(NRC), Gizza, Egypt.
Summary of my Ph.D work:
Title of the thesis:“Comparative biochemical profiling of salinity sensitive and tolerant cultivars of rice and analyzing the role of OsDREB2A transcription factor in conferring stress tolerance”.
Salinity is a severe and ever-increasing constraint on the productivity of agricultural crops. High concentrations of salts in the soil have a strong inhibitory effect on the growth and harvestable yield of all crop species. Although some prokaryotic species produce enzymes and general metabolic machinery capable of functioning in the presence of very high levels of NaCl, yet, no crop plants are known to have this capability. This does not exclude the possibility of individual enzymes, or entire metabolic pathways, having evolved to function in the presence of high sodium in halophytes, so we studied the comparative metabolite profiling to characterize the cellular response of rice plants for Proline, glutathione, ascorbate and hydrogen peroxide levels for sensitive and tolerant rice varieties (IR64 and Pokkali) , and Na K ions content too. We found higher content of proline and activity of H2O2 scavenging enzymes, glutathione and ascorbate in Pokkali as compared to IR 64. Moreover, the characterization of enzyme involved in ROS scavenging and GSH homeostasis like SOD, APX, POX, CAT, GR, GPX, Gly I, Gly II illustrate that cultivar Pokkali possessed more efficient scavenging system of reactive oxygen species defense system specially with SOD, CAT, POX.
Many genes are induced by both drought and cold stress suggesting the existence of crosstalk between the drought and cold stress signaling pathway. A combinatorial control among different promoter elements and transcription factors for a given promoter seems to be important, the analysis of those enzymes gene promoters indicate that the DRE elements is existing in most of all those genes specially POX, APX, GPX and SOD and more in Na+ /H+ antiporter. For that we selected the DREB2 gene which expression is responsive to dehydration and high salt stress and we believe that it has role to play in conferring stress tolerance. We isolate it from tolerant rice variety then transfer it into a sensitive variety using Agrobacterium mediated transformation under control of CaMV 35S promoter. We found that the OsDREB2A overexpressed rice plant is tolerant to salt and drought. We are still analyzing the antisense plant to find out the down stream genes which are regulated by OsDREB2A. We have studied the OsDREB2A recombinant protein and found the specificity of binding to the DRE core sequence elements GCCGAC more than ACCGAC using gel shift assay, also the flanking region of the same cis- element ACCGAC from its own promoter my play a role for strong binding. Also protein phosphorylation assay is going on to check the phosphorylation ability and if there is any activation/deactivation. Transcriptional analysis has shown early and up regulation of OsDREB2A gene under salt, drought and ABA treatments. We isolate OsDREB2A promoter 1.5kb from Pokkali rice variety and found that contain several important boxes in addition to DRE element and the result showed that this OsDREB2A gene is self regulated.
Acquired Skills:
Molecular Biology Skills
Ø Spectrophotometric enzymes assay.
Ø Isolation of nucleic acids (Genomic DNA, RNA and Plasmid DNA)
Ø Nuclear protein Extraction.
Ø Electrophoretic mobility shift assay (EMSA).
Ø Expression analysis by Northern blotting.
Ø PCR based and conventional cloning into suitable binary vector.
Ø Agrobacterium mediated plant transformation (conventional and In-planta)
Ø Copy number determination by Southern blotting.
Ø Protein isolation and purification using affinity chromatography.
Ø Cloning into expression vectors.
Bioinformatics Skills
Ø Annotation of sequences (BLAST).
Ø Multiple alignments by clustalW.
Ø Software’s like Mac Vector, for evolutionary and other analysis.
Ø Promoter analysis (PLACE).
Ø Primer designing by web based software’s like FastPCR, bioinformatics.
Ø PCR machine
Ø Spectrophotometer
Ø Phosphoimager.
Ø Gel documentation.
Personal Details:
Date of Birth : 1st December 1973
Sex : Male
Nationality : Egyptian
Marital Status : Married
Languages known: English, Arabic
National Research Center (NRC).
El-Bhooth St., Dokki, Giza, Egypt.
Elshabrawi H. M. Phd thesis .Comparative Biochemical Profiling of Salinity Sensitive and Tolerant Cultivars of Rice and Analyzing the Role of OsDREB2A Transcription Factor in Conferring Stress Tolerance. India
El-Shabrawi, H. M., Kumar B. and Sopory S. K. (2008). Comparative biochemical profiling between sensitive and salt tolerant rice. (Manuscript in preparation).
El-Shabrawi, H. M., Kual T, Redy M. K. and Sopory S. K. (2008). Transcriptional regulation activity of DREB2A transcription factor from indica rice under salt stress.
(Manuscript in preparation).
Prof. S. K. Sopory
Professor,
Leader of Plant Molecular Group,
International Center for Genetic Engineering and Biotechnology (ICGEB),
Aruna Asaf Ali Marg 110067 New-Delhi, India.
Dr. Mukherjee Sunil Kumar,
AssistantProfessor,
Plant Molecular Group,
International Center for Genetic Engineering and Biotechnology (ICGEB),
Aruna Asaf Ali Marg 110067 New-Delhi, India
Dr. N. Tutiga
Associate Professor,
Plant Molecular Group,
International Center for Genetic Engineering and Biotechnology (ICGEB),
Aruna Asaf Ali Marg 110067 New-Delhi, India
Dr. AFIF HASSAIRI
Address: Centre of Biotechnology at Sfax
Route Sidi Mansour Km6 Sfax
BP"K" 3038 Sfax TUNISIA
Laboratory of "Plant Molecular biology"
Mobile : 216 98 48 86 05
Tel: 216 74 440816 (post 1046)
Fax: 216 74 440818
Prof. F. M. El-Fiki
Professor,
Head of Biotechnology Department, Faculty of Agriculture, Al-Azher Unvi.
Naser City, Cairo, Egypt.
Prof. M. K. El-Baher
Professor,
Head of Plant Biotechnology Department, Genetic Engineering and Biotechnology
Division, National Research Center (NRC), El-Bhooth St., Dokki, Giza, Egypt.