Patulin is one of the most important mycotoxins that contaminates fruit-derived products and causes acute or chronic toxicity in humans. However, the methods to mitigate patulin through adsorption, irradiation and chemical modification suffer potential limitations such as safety issues, loss of nutritional quality. Biodegradation based on microbial enzymes is considered to be one of the promising ways for controlling patulin contamination.
Recently, a research team led by Prof. Li Boqiang from the Institute of Botany of the Chinese Academy of Sciences (CAS) has developed a high efficiency, stability, safety and easy separation immobilized enzyme preparation that can degrade patulin in contaminated apple juice and did not cause any adverse effects on the juice quality. This study was published in Journal of Hazardous Materials.
The researchers previously identified a short-chain dehydrogenase/reductase (CgSDR) which has the function of degrading patulin. In the present study, a novel patulin-degrading enzyme preparation was further constructed by covalently linking CgSDR to dopamine/polyethyleneimine co-deposited magnetic Fe3O4 particles.
The researchers found that the immobilized enzyme possesses combined effects of patulin adsorption by Fe3O4 particles and biological degradation by CgSDR, and exhibited a detoxification rate of 100% in phosphate-buffered saline and a detoxification rate of more than 80% in apple juice.
Moreover, they demonstrated that compared with the free enzyme, the immobilized CgSDR substantially improved thermal and storage stabilities, proteolysis resistance, and reusability.
“Notably, this novel patulin-degrading enzyme can be easily separated from apple juice through external magnetic filed, and retains more than 50% detoxification rate after seven consecutive cycles of reuse, which significantly reduces the costs in future application,” said Prof. TIAN Shiping, one of the correspondent authors.
Application of a magnetically recoverable enzyme formulation in patulin detoxification in fruit derived products
Article Link: https://doi.org/10.1016/j.jhazmat.2023.130986
Contact:
LI Boqiang
Institute of Botany, the Chinese Academy of Sciences
Email: bqli@ibcas.ac.cn
Patulin is one of the most important mycotoxins that contaminates fruit-derived products and causes acute or chronic toxicity in humans. However, the methods to mitigate patulin through adsorption, irradiation and chemical modification suffer potential limitations such as safety issues, loss of nutritional quality. Biodegradation based on microbial enzymes is considered to be one of the promising ways for controlling patulin contamination.
Recently, a research team led by Prof. Li Boqiang from the Institute of Botany of the Chinese Academy of Sciences (CAS) has developed a high efficiency, stability, safety and easy separation immobilized enzyme preparation that can degrade patulin in contaminated apple juice and did not cause any adverse effects on the juice quality. This study was published in Journal of Hazardous Materials.
The researchers previously identified a short-chain dehydrogenase/reductase (CgSDR) which has the function of degrading patulin. In the present study, a novel patulin-degrading enzyme preparation was further constructed by covalently linking CgSDR to dopamine/polyethyleneimine co-deposited magnetic Fe3O4 particles.
The researchers found that the immobilized enzyme possesses combined effects of patulin adsorption by Fe3O4 particles and biological degradation by CgSDR, and exhibited a detoxification rate of 100% in phosphate-buffered saline and a detoxification rate of more than 80% in apple juice.
Moreover, they demonstrated that compared with the free enzyme, the immobilized CgSDR substantially improved thermal and storage stabilities, proteolysis resistance, and reusability.
“Notably, this novel patulin-degrading enzyme can be easily separated from apple juice through external magnetic filed, and retains more than 50% detoxification rate after seven consecutive cycles of reuse, which significantly reduces the costs in future application,” said Prof. TIAN Shiping, one of the correspondent authors.
Application of a magnetically recoverable enzyme formulation in patulin detoxification in fruit derived products
Article Link: https://doi.org/10.1016/j.jhazmat.2023.130986
Contact:
LI Boqiang
Institute of Botany, the Chinese Academy of Sciences
Email: bqli@ibcas.ac.cn